Click on the Name of Any Core Laboratory for More Information
on that Core.
|Administrative Core: Directed
by Michael K. Skinner, this core integrates the projects by organizing
the routine seminars and workshops, as well as the annual retreat.
The Administrative Core will oversee all fiscal management of
the program and facilitate university and agency interactions.
This core provides some general administrative services, in particular
the interactions both in regards to budgets and services of the
|Histology Core: Directed
by Kwan Hee Kim, this core provides services to obtain histological
sections and related methods to the investicators in the center.
The core also provides training and individualized procedure
development for tissue preparation, immunohistochemistry, and
in situ hybridization. The digital image analysis facility is
available to all investigators.
Assay Core: Directed by Derek McLean. The core is an assay
core to analyze hormone (i.e. steroid and gonadotropin) levels in
the animals exposed to endocrine disruptors. Radioimmunoassays are
routinely done for steroids and the reagents are available for gonadotropins
of several of the species of interest. The service provided are
to perform the assays on samples provided by the investigators.
Toxicology Core: Directed by Rolf Ingermann. This core
is the assay of toxins and metabolites in tissues and biological
fluids. The objective is to allow investigators to analyze toxin
levels and metabolites in animals exposed to endocrine disruptors.
A regional toxicology laboratory a the University of Idaho is
available that performs all the needed gas chromatography and
assays. The core laboratory coordinates the use of this regional
toxicology laboratory so that this service is available to all
|Aquaculture Core: Directed by Gary
Thorgaard and Ken Cain, the aquaculture core has been established
to insure proper and consistent handling and rearing of the animals
among the projects. The aquaculture core will be composed of two parts.
One component of the core will be housed at Washington State University.
This facility will be composed of a number of recirculating systems
and will be used to support small-scale laboratory experiments. The
oversight for this part of the aquaculture core will be provided by
Gary Thorgaard. The second component will be at the University of
Idaho aquaculture. The University of Idaho core facility will be used
to support broodstock rearing and the more long-term, applied experiments.
The oversight for this part of the core will be provided by Ken Cain,
director of the facility.
|Transgenic Core: Directed
by Michael Skinner, the Transgenic Core aids in providing, maintaining,
and identifying transgenic and knockout mice at a subsidized
cost to investigators within the Center for Reproductive Biology.
Therefore, the objectives of the center are: 1) Facilitate acquisition
of transgenic and knockout animals from outside vendors at reduced
costs; 2) Provide training to Genotype transgenic or knockout
mice; 3) Provide expertise to maintain and establish initial
transgenic or knockout founders for colonies; 4) Provide initial
space for transgenic and knockout colonies.
|Molecular Biology Core: Co-Directed
by Michael Griswold and Derek Pouchnik, this Core provides DNA sequencing,
protein sequencing, oligonucleotide synthesis, peptide synthesis,
and some information services to member of the Center for Reproductive
Biology. A state of the art core laboratory with all the required
equipment is available.
|Animal Reproduction Core: Directed by
Derek McLean. The Animal Reproduction Core provides centralized
facilities, services and expertise on handling and housing laboratory
and domestic animals used by members of the Center for Reproductive
Biology in accomplishing their research objectives. Services
such as animal handling, surgical technique, cannulation, blood
collection, tissue collection at either the university's abattoir
or commercial packing plants will be aided by this core. Technical
advice on animal care and scientific design and coordination
of services will be provided by the Core Director and a core
technician associated with the Director's laboratory. The facilities,
equipment, and animals of this core are incorporated into the
Department of Animal Sciences animal facilities to capitalize
on the expertise and efficiency of this already established unit.
|Genomics Core: Co-Directed by Michael Griswold
and Derek Pouchnik, the Genomics Core provides state of the art genomics
micro-array technology equipment. The core laboratory provides access
to the both an Affimetrix Micro-Array apparatus and data analysis
system, as well as a custom array apparatus for glass slide array
of investigator initiated arrays. The Core has the bio-informatics
support for the procedures performed and assists the investigators
in data analysis. Technical support is provided to perform the analysis
on the Affimetrix chips and in the production of the custom arrays.
| Proteomics Core: Directed by James
E Bruce, the Proteomics Core provides sstate-of-the art proteomics
research on campus and enable much greater insight in many biological
research programs at WSU. The objectives of this core are to
provide access to technology and expertise capable of measuring
relative quantitation of proteins from complex mixtures such
as cell lysates, as well as facilitate the identification for
those proteins found to be up- or down-regulated in response
to particular stimulus, disease or treatment of interest. These
capabilities will significantly extend the understanding of many
research programs by providing systems-level comprehension for
cases where little or nothing is known about the underlying biology
for a physical process, as well as provide a much broader view
of functional significance for specific genes currently known
to be involved in pathways that may represent only a small subset
of actual functionality. In addition, this core lab will serve
as a resource for consultation, education and training in current
proteomics technology, help further accelerate biological research
programs at WSU, and better educate WSU graduates for success
in the increasingly competitive biotechnology sector.
Bioinformatics Core: Directed by Michael
Skinner provides computational expertise for genomic and proteomic
research studies. Staff can provide investigators with recommendations
for hardware and software necessary to perform data analysis, storage,
and management. The Bioinformatics core provides investigators with
centralized DNA/Protein, microarray, proteomics data analysis services.
The core consists of secure high performance computers/servers and
storage devices linked to the genomics, proteomics and image analysis
core. The data generated by these core facilities is seamlessly
integrated for analysis by most commonly used analysis packages.
The individual users can also link to central servers to access
their data for analysis. WSU Bioinformatics core operates within
an academic 'hybrid zone' involving Life, Computer Sciences and
Engineering. Bioinformatics core services will be accessed through
the The WSU-bioinformatics web site with links to the servers, mirror
sites for WWW sites for DNA/protein analysis and centralized servers
including a Storage Area Network (SAN) (web-based client/server
|Molecular Cytogenetics Core: Directed
by Lisa Shaffer, this core provides (1) routine karyotype analysis
on the investigator's established human cell lines or human clinical
samples; (2) molecular cytogenetic analysis (fluorescent in situ
hybridization, FISH) using the investigator's clone and either
the investigator's cell lines or clinical samples, or on normal
control cell lines from the Core; (3) 24-color karyotyping of
human cell lines to identify complex rearrangements; (4) mapping
of human large-insert clones from the investigator's laboratory
to determine a map position in normal control cell lines from
the Core; (5) assist in identifying human large-insert clones
for use in the Core; (6) lymphoblastic EBV transformation of
human peripheral blood samples to establish permanent cell lines;
(7) store investigator's established cell lines, provide growing
cultures back to investigators, and provide fixed cell pellets
back to investigators. The Core is willing to engage in karyotype
analysis of non-human cell lines and clinical specimens if investigators
are willing to supply normal examples from several individuals
from a species in order for the Core to gain expertise prior
to performing karyotypic analysis on clinical specimens. Future
work could extend into FISH on non-human cell lines using clones
and cell lines provided from the investigators.
|Flow Cytometry Core: Directed
by Dr. William Davis, this core provides members with access
to the Flow Cytometry Core facility and provide users with personal
support for cell sorting, operation of equipment and data analysis.
Housed in the newly built Animal Disease and Biotechnology Facility,
the College of Veterinary Medicine operates this facility. This
facility currently has three flow cytometers: FACScan, FACSort,
FACSVantage (Becton Dickinson Immunocytometry Systems). The
FACScan is a simple flow cytometer. It has a single laser and
can analyze five separate parameters (side and forward scatter
light scatter and 3 fluorescent markers). The FACSort has two
lasers and can analyze and sort cells based on 6 separate parameters
(side and forward scatter, and 4 fluorescent markers). The FACS
Vantage SE is a high speed cell sorter. It has two lasers and
can analyze and sort based on 8 separate parameters (side and
forward scatter and 6 fluorescent markers). All flow cytometers
have internet access and are equipped with software for data
acquisition and analysis. There are also two separate computer
workstations for data analysis
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