Sertoli Cell Function
For the past 15 years my research has been directed towards the understanding of mammalian spermatogenesis. We have concentrated our efforts on the delineation of the influence of the Sertoli cells on the process of germinal cell differentiation and maturation. Much of our interest has focused on the glycoproteins which are secreted by Sertoli cells and interact with a variety of germinal cells. We utilize a variety of molecular approaches in attempts to characterize the Sertoli-germ cell interaction at the molecular level. In addition, we are characterizing gene products and regulatory mechanisms that are unique to the somatic cells. For example, we identified transferrin as a Sertoli cell secretion product and described a process whereby Sertoli cells deliver iron from the testicular lymph to the spermatocytes and round spermatids. Similar types of studies on other Sertoli cell products are currently under way.
Dan Johnston, a Postdoc from Dr. Griswold's laboratory, is dissecting seminiferous tubules for germ cell isolation.
Koubova J, Menke DB, Zhou Q, Capel B, Griswold MD, Page DC. 2006 Retinoic acid regulates sex-specific timing of meiotic initiation in mice. Proc Natl Acad Sci USA. 103(8):2474-9.
Griswold, M. D. and Kim, J.-S. (2001) Site-specific Methylation Alters DNA-Protein Interactions and Prevents Follicle-stimulating Hormone Receptor Gene Transcription. Biol Reprod. Feb;64(2):602-610
Lok, S., Johnston. D., Conklin, D., Lofton-Day, C., Adams,
R., Jelmberg, A., Whitmore, T., Schrader, S., Griswold, M.,
and Jaspers, S. (2000) Identification of INSL6, a new member
of the insulin superfamilty that is expressed in the testis.
Biol Reprod. 2000 62(6):1593-9.
Russell, L. and Griswold, M. D. (2000) Spermatogonial
Transplantation- An Update for the Millenium Mol Cell Endocrinol.
2000 Mar 30;161(1-2):117-20.
Komada, M., McClean, D., Griswold, M. D., Russell,
L., and Soriano, P. (2000) E-Map-115, encoding a microtubule-associated
protein, is a retinoic acid inducible gene required for spermatogenesis.
Genes Dev. Jun 1;14(11):1332-1342.
Johnston, D., Russell, L., and Griswold, M. D. (2000)
Advances in Spermatogonial Stem Cell Transplantation, Reviews
in Reproduction submitted
Braun, K. W., Tribley, W. A., Griswold, M. D., and
Kim, K. (2000) FSH Inhibits tRA-Induced Retinoic Acid Receptor
a Nuclear Localization and Transcriptional Activation in Mouse
Sertoli Cells. J Biol. Chem, 275: 4145-4151
Griswold, M.D. (2000) Editorial: What can spermatogonial
transplants teach us about male reproductive biology? Endocrinology
Boettger-Tong, H., Johnston, D., Griswold, M. D., and
Bishop, C. (2000) Jsd (Juvenile Spermatogonial Depletion) Mutant
Seminiferous Tubules are capable of Supporting Transplanted Spermatogenesis.
Biol. Reprod. In press
McClean, D., Johnston, D., Russell, L., and Griswold, M.
D. (2000) Germ Cell Transplantation and Testicular function,
Trends in Endocrinology and Metabolism in press.
Tribely, W., Kim, J. and Griswold, M. (2000) Mechanisms
involved in the homologous down regulation of the Follicle Stimulating
hormone receptor gene in Sertoli cells. submitted
Bailey, R. and Griswold, M. D. (1999) Clusterin in the Male
Reproductive System: Localization and Possible Function. Mol.
Cell. Endocrinology 151:5-16.
Russell, L. D. and Griswold, M. D. (1999) Transgenic
Technology in Animal Reproductive Processes: Methods, Interpretation
and Contributions. In: Molecular Biology in Reproductive Medicine,
(B. C. J. M. Fauser, A. J. Rutherford, J. F. Strauss, A. Van
Steirteghem, eds.) Parthenon Publishing Group, New York, pp.
49-66.Griswold. M. D. (1998) The central role of Sertoli
cells in spermatogenesis. Seminars in Developmental Biology
Semin Cell Dev Biol. 1998 Aug;9(4):411-6.
Sutton, K., Maiti, S., Tribely, W., Lindsey, J., Meistrich,
M., Bucana, C., Sanborn, B., Joseph, D., Griswold, M.,
Cornwall, G., and Wilkenson, M. (1998) Androgen regulation of
the PEM homeodomain gene in mice and rat Sertoli and epididymal
cells. J. Andrology 19:21-30.
Maguire, S., Tribley, W. A. and Griswold, M. D. (1997) Follicle-stimulating hormone regulates expression of FSH receptor mRNA in cultured Sertoli cells and in hypophysectomized rats. Biol. Reprod.56: 1106-1111.
Goetz, T., Lloyd, T. and Griswold, M. D. (1997) Role of the E box and Initiator region in the Expression of the Rat Follicle Stimulating Hormone Receptor. J. Biol. Chem. 271: 33317-33324
Graf, K., Dias, J. and Griswold, M. D. (1997) Decreased spermatogenesis as a result of an induced autoimmune reaction directed against the gonadotropin receptors in male rats, J. Andrology 18: 174-185.
Law, G. Lynn, McGuinness, Michael P., Linder, Carol C. and Griswold, Michael D. (1997) Expression of apolipoprotein E mRNA in the epithelium and interstitium of the testis and the epididymis. J. Androl. 18: 32-42.
Clark, A. and Griswold, M. D. (1997) Expression of clusterin/sulfated glycoprotein-2 under conditions of heat stress in rat Sertoli cells and a mouse Sertoli cell line. J. Androl. 18 (3): 257-263.
Clark, A., Maguire, S. M. and Griswold, M. D. (1997) Accumulation of clusterin/sulfated glycoprotein-2 in degenerating pachytene spermatocytes of adult rats treated with methoxyacetic acid. Biol. Reprod. 57: 837-846.