Dean & Regents Professor School of Molecular Biosciences Washington State University Pullman, WA 99164-4660   Phone: 509-335-6281 FAX: 509-335-9688 E-mail: griswold@mail.wsu.edu    Ph.D., 1969 University of Wyoming RESEARCH INTERESTS: Sertoli Cell Function RESEARCH SUMMARY: For the past 15 years my research has been directed towards the understanding of mammalian spermatogenesis. We have concentrated our efforts on the delineation of the influence of the Sertoli cells on the process of germinal cell differentiation and maturation. Much of our interest has focused on the glycoproteins which are secreted by Sertoli cells and interact with a variety of germinal cells. We utilize a variety of molecular approaches in attempts to characterize the Sertoli-germ cell interaction at the molecular level. In addition, we are characterizing gene products and regulatory mechanisms that are unique to the somatic cells. For example, we identified transferrin as a Sertoli cell secretion product and described a process whereby Sertoli cells deliver iron from the testicular lymph to the spermatocytes and round spermatids. Similar types of studies on other Sertoli cell products are currently under way. Dan Johnston, a Postdoc from Dr. Griswold's laboratory, is dissecting seminiferous tubules for germ cell isolation.   REPRESENTATIVE PUBLICATIONS Koubova J, Menke DB, Zhou Q, Capel B, Griswold MD, Page DC. 2006 Retinoic acid regulates sex-specific timing of meiotic initiation in mice. Proc Natl Acad Sci USA. 103(8):2474-9. Lee TH, Yi W, Griswold MD, Zhu F, Her C. 2006 Formation of hMSH4-hMSH5 heterocomplex is a prerequisite for subsequent GPS2 recruitment. DNA Repair (Amst).5(1):32-42. Li Y, Putnam-Lawson CA, Knapp-Hoch H, Friel PJ, Mitchell D, Hively R, Griswold MD. 2005 Immunolocalization and Regulation of Cystatin 12 in Mouse Testis and Epididymis. Biol Reprod. 73(5):872-80. Meng J, Holdcraft RW, Shima JE, Griswold MD, Braun RE. 2005 Androgens regulate the permeability of the blood-testis barrier. Proc Natl Acad Scie USA. 102(46):16696-700. Li Y, Friel PJ, McLean DJ, Griswold MD. 2003 Cystatin E1 and E2, New Members of Male Reproductive Tract Subgroup Within Cystatin Type 2 Family. Biol Reprod. 69(2):489-500. Anway MD, Li Y, Ravindranath N, Dym M, Griswold MD. 2003 Expression of testicular germ cell genes identified by differential display analysis. J Androl. 24(2):173-84. Griswold, M. D. and Kim, J.-S. (2001) Site-specific Methylation Alters DNA-Protein Interactions and Prevents Follicle-stimulating Hormone Receptor Gene Transcription. Biol Reprod. Feb;64(2):602-610 McClean, D., Johnston, D., Russell, L., and Griswold, M. D. (2001) Germ Cell Transplantation and Testicular function, Trends in Endocrinology and Metabolism 12:16-21. Tribely, W., Kim, J. and Griswold, M.D. (2001) Mechanisms involved in the homologous down regulation of the Follicle Stimulating hormone receptor gene in Sertoli cells. Mol. Cell. Endo. 173:95-107. Johnston DS, Russell LD, Friel PJ, Griswold MD. (2001) Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation. Endocrinology. Jun;142(6):2405-8. Lok, S., Johnston. D., Conklin, D., Lofton-Day, C., Adams, R., Jelmberg, A., Whitmore, T., Schrader, S., Griswold, M., and Jaspers, S. (2000) Identification of INSL6, a new member of the insulin superfamilty that is expressed in the testis. Biol Reprod. 2000 62(6):1593-9. Russell, L. and Griswold, M. D. (2000) Spermatogonial Transplantation- An Update for the Millenium Mol Cell Endocrinol. 2000 Mar 30;161(1-2):117-20. Komada, M., McClean, D., Griswold, M. D., Russell, L., and Soriano, P. (2000) E-Map-115, encoding a microtubule-associated protein, is a retinoic acid inducible gene required for spermatogenesis. Genes Dev. Jun 1;14(11):1332-1342. Johnston, D., Russell, L., and Griswold, M. D. (2000) Advances in Spermatogonial Stem Cell Transplantation, Reviews in Reproduction submitted Braun, K. W., Tribley, W. A., Griswold, M. D., and Kim, K. (2000) FSH Inhibits tRA-Induced Retinoic Acid Receptor a Nuclear Localization and Transcriptional Activation in Mouse Sertoli Cells. J Biol. Chem, 275: 4145-4151 Griswold, M.D. (2000) Editorial: What can spermatogonial transplants teach us about male reproductive biology? Endocrinology 141:857-858 Boettger-Tong, H., Johnston, D., Griswold, M. D., and Bishop, C. (2000) Jsd (Juvenile Spermatogonial Depletion) Mutant Seminiferous Tubules are capable of Supporting Transplanted Spermatogenesis. Biol. Reprod. In press McClean, D., Johnston, D., Russell, L., and Griswold, M. D. (2000) Germ Cell Transplantation and Testicular function, Trends in Endocrinology and Metabolism in press. Tribely, W., Kim, J. and Griswold, M. (2000) Mechanisms involved in the homologous down regulation of the Follicle Stimulating hormone receptor gene in Sertoli cells. submitted Bailey, R. and Griswold, M. D. (1999) Clusterin in the Male Reproductive System: Localization and Possible Function. Mol. Cell. Endocrinology 151:5-16. Russell, L. D. and Griswold, M. D. (1999) Transgenic Technology in Animal Reproductive Processes: Methods, Interpretation and Contributions. In: Molecular Biology in Reproductive Medicine, (B. C. J. M. Fauser, A. J. Rutherford, J. F. Strauss, A. Van Steirteghem, eds.) Parthenon Publishing Group, New York, pp. 49-66.Griswold. M. D. (1998) The central role of Sertoli cells in spermatogenesis. Seminars in Developmental Biology Semin Cell Dev Biol. 1998 Aug;9(4):411-6. Sutton, K., Maiti, S., Tribely, W., Lindsey, J., Meistrich, M., Bucana, C., Sanborn, B., Joseph, D., Griswold, M., Cornwall, G., and Wilkenson, M. (1998) Androgen regulation of the PEM homeodomain gene in mice and rat Sertoli and epididymal cells. J. Andrology 19:21-30. Maguire, S., Tribley, W. A. and Griswold, M. D. (1997) Follicle-stimulating hormone regulates expression of FSH receptor mRNA in cultured Sertoli cells and in hypophysectomized rats. Biol. Reprod.56: 1106-1111. Goetz, T., Lloyd, T. and Griswold, M. D. (1997) Role of the E box and Initiator region in the Expression of the Rat Follicle Stimulating Hormone Receptor. J. Biol. Chem. 271: 33317-33324 Graf, K., Dias, J. and Griswold, M. D. (1997) Decreased spermatogenesis as a result of an induced autoimmune reaction directed against the gonadotropin receptors in male rats, J. Andrology 18: 174-185. Law, G. Lynn, McGuinness, Michael P., Linder, Carol C. and Griswold, Michael D. (1997) Expression of apolipoprotein E mRNA in the epithelium and interstitium of the testis and the epididymis. J. Androl. 18: 32-42. Clark, A. and Griswold, M. D. (1997) Expression of clusterin/sulfated glycoprotein-2 under conditions of heat stress in rat Sertoli cells and a mouse Sertoli cell line. J. Androl. 18 (3): 257-263. Clark, A., Maguire, S. M. and Griswold, M. D. (1997) Accumulation of clusterin/sulfated glycoprotein-2 in degenerating pachytene spermatocytes of adult rats treated with methoxyacetic acid. Biol. Reprod. 57: 837-846.