You are interested in cloning and sequencing the genome of a bacteria that grows in hot springs in Yellowstone National Park. The genome size is about 5 MB (million bases).

1. If the average size of BAC inserts is 150 kb, how many BACs would be needed to cover the genome?
2. If the inserts on average had 50% overlap with other BACs (to allow construction of Contigs) how many inserts would be needed?
3. How might you go about using restriction enzymes for generating the large inserts used in such a library? What enzymes (e.g., 4, 6 or 8 base recognition) might you choose, and why? Would a partial digestion with another type of enzyme be a viable option?
4. Describe at least two ways in which you might interpret the data as you sequence the genome.

What results might you obtain if you used a cDNA clone (insert 1.8 kb in size) to probe a BAC (genomic) library (average insert size 150 kb), and what additional information might you obtain from analyzing the BAC clone(s) that you isolate?

Also, problems 10.3 (note: tet stands for the antibiotic tetracycline), 10.4, 10.7, 10.8 and 11.3