The USDA-ARS Western Wheat Quality Laboratory has established this puroindoline genotype database as a service to wheat researchers, breeders and the public. The puroindoline genes, puroindoline a and puroindoline b, represent the Hardness gene on 5DS. In hexaploid wheat, soft kernel texture (Hardness) is the wild type, whereas hard texture (hardness) results from any one of several mutations in the puroindolines. These mutations have been assigned allelic designations as per the revised .Guidelines for Nomenclature of Biochemical/Molecular Loci in Wheat and Related Species. (McIntosh et al. 1995)(8).