[ Graphics OFF ]
HOME ABOUT US TOUR STAFF LINKS CONTACT US SITE MAP
WHEAT ANALYSIS SYSTEM REGIONAL QUALITY DATA PUROINDOLINE PUBLICATIONS VARIETY QUALITY SCORES BREEDERS LOGIN PNW WQC INFO
Recent Publications
Abstract
Wheat grain hardness results from highly conserved mutations in the friabilin components Puroindoline a and b. Proceedings of the National Academy of Sciences 95:6262-6266
Giroux,M.J. and Morris,C.F.
USDA/ARS Western Wheat Quality Lab
"Soft" and "hard" are the two main market classes of wheat (Triticum aestivum L.) and are distinguished by expression of the Hardness gene. Friabilin, a marker protein for grain softness (Ha), consists of two proteins, puroindoline a and b (pinA and pinB, respectively). We previously demonstrated that a glycine to serine mutation in pinB is linked inseparably to grain hardness. Here, we report that the pinB serine mutation is present in 9 of 13 additional randomly selected hard wheats and in none of 10 soft wheats. The four exceptional hard wheats not containing the serine mutation in pinB express on pinA, the remaining component of the marker protein friabilin. The absence of pinA protein was linked inseparably to grain hardness among 44 near-isogenic lines created between the soft variety Heron and the hard variety Falcon. Both pinA and pinB apparently are required for the expression of grain softness. The absence of pinA protein and transcript and a glycine-to-serine mutation in pinB are two highly conserved mutations associated with grain hardness, and these friabilin genes are the suggested tightly linked components of the Hardness gene. A previously described grain hardness related gene termed "GSP-1" (grain softness protein) is not controlled by chromosome 5D and is apparently not involved in gain hardness. The association of grain hardness with mutations in both pinA and pinB indicates that these two proteins alone may function together to effect grain softness. Elucidation of the molecular basis for grain hardness opens the way to understanding and eventually manipulating this wheat endosperm property.
Printable Version
USDA Nondiscrimination Statement | Statements and Disclaimers | WSU Copyright | WSU Disclaimer and Freedom of Information Statement
1997 - 2010 USDA/ARS Western Wheat Quality Laboratory - Webmaster