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Abstract
Expression of wild-type pinB sequence in transgenic wheat complements a hard phenotype Theoretical and Applied Genetics 105:870-877
Beecher,B., Bettge,A., Smidansky E. and Giroux, M.J.
Dept. Of Plant Sciences and Plant Pathology, Ag. BioSci. Fac., Montana State Univ., Bozeman, MT 59717 Dept. Of Agronomy and Horticulture, Univ. of Nebraska, Lincoln, NB 68583 USDA-ARS Western Wheat Quality Lab, FSHN East, Washington State Univ., Pullman
Wheat grain hardness is a major factor in wheat end-product quality. Grain hardness in wheat affects such parameters as milling yield, starch damage and baking properties. A single locus determines whether wheat is hard or soft textured. This locus, termed Hardness (Ha), resides on the short arm of chromosome 5D. Sequence alterations in the tryptophan-rich proteins puroindoline a and b (PINA and PINB) are inseparably linked to hard textured grain, but their role in endosperm texture has been controversial. Here, we show that the pinB-D1b alteration, common in hard textured wheats, and be complemented by the expression of wild-type pinB-D1a in transformed plants. Transgenic wheat seeds expressing wild-type pinB were soft in phenotype, having greatly increased friabilin levels, and greatly decreased kernel hardness and damaged starch. These results indicate that the pinB-D1b alteration is most likely the causative Ha mutation in the majority of hard wheats.
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