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Abstract
Evaluation of maternal parent and puroindoline allele on kernel texture in a reciprocal cross between two hard spring wheat cultivars Euphytica 141:121-127
Gedye,K.R.,Bettge,A.D.,King,G.E.,Morris,C.F.
Kernel texture is an important characteristic for both the milling and the end-use quality of wheat (Triticum aestivum L.). Gene sequence variation and mutations to the two puroindoline genes (Pina and Pinb), located at the Ha locus on chromosome 5DS, account for the majority of variation in wheat kernel texture. Other factors also influence kernel texture, including effects associated with different maternal parent backgrounds. To investigate the effect of two hard puroindoline alleles in different maternal backgrounds, a population of 228 recombinant inbred lines (RILs) derived from a reciprocal cross between two wheat cultivars ‘ID377s’ (Pina-D1b/Pinb-D1a) and ‘Klasic’ (Pina-D1a/Pinb-D1b) were examined in two succeeding generations (F7 & F8). Kernel texture was determined using the Single Kernel Characterization System (SKCS) and the RIL puroindoline haplotype was identified by the sequence-specific PCR amplification of each gene. Analysis of variance identified a significant (P ? 0.001) effect of the maternal parent and puroindoline mutation on kernel texture. RILs containing the Pina- D1b mutation were significantly harder than lines containing the Pinb-D1b mutation. RILs which had Klasic as the maternal parent were significantly harder than those which had ID377s as the maternal parent. When the maternal parent and puroindoline allele were analyzed in combination, RILs derived from Klasic as the maternal parent and the Pina-D1b allele were significantly harder (P ? 0.001) than those containing the same allele but ID377s as the maternal parent. The same occurred for RILs containing the Pinb-D1b allele, lines with Klasic as the maternal parent were harder than lines with ID377s as the maternal parent. These results corroborate the harder phenotype of the Pina-D1b allele and indicate a significant maternally-inherited contribution to kernel texture variation.
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