Describe how you would experimentally go about identifying whether the following phenomena might be associated with regulation of expression of a gene (B-mal) coding for an enzyme in a frog. You have isolated genomic DNA and messenger RNA from five tissues (liver, kidney, muscle, heart and spleen) at two different developmental stages (tadpole and adult) in a species of frog.

1. Gene amplification.
2. DNA splicing changes (genomic level changes)
3. DNA methylation changes
4. Differential splicing of transcribed RNA
5. Varying levels of messenger RNA

Also: Problems 11.4, 11.6 and 11.12.