Genetics 301 Quiz #10
Spring 2003
Class week 13

 

  1. You believe that differences in gene expression for a gene in mice may be related to differences in splicing of the messenger RNA between tissues. How would you go about testing your hypothesis about differential splicing? (5 pts.)

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    The best approach would be to isolate RNA for both tissues, prepare cDNAs,, and sequence the cDNAs to determine if there were differences between tissues. These results could be related to the genomic DNA sequences which indicate full sequence of the gene and the location of introns.

     

     

     

     

     

     

  2. If the level of glucose in E. coli cells is low, the level of cyclic AMP is normally high. Describe how this can affect expression of the lac operon genes and the mechanism by which it does so (5 pts.).

 

Cyclic AMP can bind to a protein called CRP (cyclic AMP receptor protein). That complex can then bind to a region near the lac promoter. Binding of the complex facilitates transcription of the operon (positive control of transcription).